分子别名 (Synonym)
SHH; Sonic Hedgehog; HHG-1; Sonic hedgehog; HLP3; HPE3; SMMCI; TPT; TPTPS
来源/表达系统 (Source)
Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag.It contains Cys24-Gly197 [Accession | Q15465].
分子量
The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
内毒素 (Endotoxin)
Less than 0.05 EU per μg by the LAL method.
纯度 (Purity)
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
制剂 (Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
重构方法 (Reconstitution)
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
存储 (Storage)
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
