Biotinylated Cynomolgus PADI4 Protein (Primary Amine Labeling)

Name: Biotinylated Cynomolgus PADI4 Protein (Primary Amine Labeling)
Brand: Puweituo Bio
SKU: PADI4-CB001

Catalog numberPADI4-CB001

E. coliMolecule · Protein-arginine deiminase type-4Molecular weight · 76.6 kDa

Product introduction

Recombinant Biotinylated Cynomolgus PADI4 Protein (Primary Amine Labeling) is expressed in E. coli with a His tag at the N-terminus. It contains amino acid residues Met1-Pro663 (UniProt accession: A0A2K5USI5).

Product details

Molecular aliases: Protein-arginine deiminase type-4; HL-60 PAD; HL60 PAD; HL-60; PAD; PAD4; PADI5; PDI5; HL60PAD; HL60
Protein length: Met1-Pro663
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 76.6 kDa same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol, 0.5mM TCEP, 1mM EDTA (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus PADI4 (Primary Amine Labeling) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Biotinylated Cynomolgus PADI4, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-PADI4 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. (QC Test)

Background

Peptidylarginine deiminase type4 (PADI4) was firstly identified as a non-MHC RA genetic risk factor. Furthermore, PADI4 risk allele possessed the association with bone damage regardless of anti citrullinated peptide antibody (ACPA) positivity in Asian RA patients. PADI4 gene codes PAD4 protein which has post-translational modification activity (citrullination). Padi4 is mainly expressed in myeloid cells and granulocytes.

References

1.Seri Y, Shoda H, Matsumoto I, Sumida T, Fujio K, Yamamoto K. [Peptidylarginine deiminase type4 (PADI4) role in immune system]. Nihon Rinsho Meneki Gakkai Kaishi. 2014;37(3):154-9. Japanese. doi: 10.2177/jsci.37.154. PMID: 24974927.

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Biotinylated Cynomolgus PADI4 Protein (Primary Amine Labeling)

Catalog numberPADI4-CB001

E. coliMolecule · Protein-arginine deiminase type-4Molecular weight · 76.6 kDa

Biotinylated Cynomolgus PADI4 Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References

Biotinylated Cynomolgus PADI4 Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References