Mouse OX40 Ligand/TNFSF4 Protein

Name: Mouse OX40 Ligand/TNFSF4 Protein
Brand: Puweituo Bio
SKU: OX40-MF001

Catalog numberOX40-MF001

MouseHEK293Molecule · OX40LMolecular weight · 42.6 kDa

Product introduction

Recombinant Mouse OX40 Ligand/TNFSF4 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Ser51-Leu198 (UniProt accession: P43488).

Product details

Molecular aliases: OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L; TXGP1; Glycoprotein Gp34
Protein length: Ser51-Leu198
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 52-56 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per ug by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Mouse OX40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Mouse OX40 Ligand is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Mouse OX-40 Ligand, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 0.91μg/ml determined by ELISA. (QC Test)

Background

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.

References

1.Al-Shamkhani A, Mallett S, Brown M H, et al. Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells[J]. Journal of Biological Chemistry, 1997, 272(8):5275-5282. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Mouse OX40 Ligand/TNFSF4 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Mouse OX40 Ligand/TNFSF4 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References