Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer Protein

Name: Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer Protein
Brand: Puweituo Bio
SKU: MAGEA10-HP002

Catalog numberMAGEA10-HP002

HumanHEK293Molecule · MAGE-A10Molecular weight · 258 kDa

Product introduction

Recombinant Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GLYDGMEHL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &GLYDGMEHL).

Product details

Molecular aliases: MAGE-A10
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer is greater than 95% as determined by SEC-HPLC.

Background

MAGE-A10 is a subtype of the Melanoma-associated antigen A (MAGE-A), a class of tumor antigens that are extensively expressed in various histological types of tumors and represents an attractive target for tumor immunotherapy. High-level expression of MAGE-A10 improved the anti-tumor immune cytotoxicity of MAGE-A10-specific CTLs in lung cancer cell lines and primary lung cancer cells.

References

1.Li Z, Guo P, Guo P, Dong K, Liu F, Wu Y, Li J, Shan B, Sang M. 5-aza-2'-deoxycytidine (DAC) treatment induces the MAGE-A10 expression and improves the cytotoxicity of MAGE-A10-specific CTLs in lung cancer cells. Transl Cancer Res. 2020 Feb;9(2):1235-1245. doi: 10.21037/tcr.2020.01.10. PMID: 35117468; PMCID: PMC8798493.

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Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer Protein

Catalog numberMAGEA10-HP002

HumanHEK293Molecule · MAGE-A10Molecular weight · 258 kDa

Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References