Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein
Brand: Puweituo Bio
SKU: MAGEA1-HB001

Catalog numberMAGEA1-HB001

HumanHEK293Molecule · MAGE-A1Molecular weight · 50.50 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &KVLEYVIKV).

Product details

Molecular aliases: MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-65 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).

Background

MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors.

References

1.Wang D, Wang J, Ding N, Li Y, Yang Y, Fang X, Zhao H. MAGE-A1 promotes melanoma proliferation and migration through C-JUN activation. Biochem Biophys Res Commun. 2016 May 13;473(4):959-965. doi: 10.1016/j.bbrc.2016.03.161. Epub 2016 Apr 1. PMID: 27045082.

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Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein

Catalog numberMAGEA1-HB001

HumanHEK293Molecule · MAGE-A1Molecular weight · 50.50 kDa

Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References