Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein
Brand: Puweituo Bio
SKU: MAGEA1-HB002

Catalog numberMAGEA1-HB002

HumanE. coliMolecule · MAGE-A1Molecular weight · 35.6 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &KVLEYVIKV).

Product details

Molecular aliases: MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC.

Background

MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors.

References

1.Wang D, Wang J, Ding N, Li Y, Yang Y, Fang X, Zhao H. MAGE-A1 promotes melanoma proliferation and migration through C-JUN activation. Biochem Biophys Res Commun. 2016 May 13;473(4):959-965. doi: 10.1016/j.bbrc.2016.03.161. Epub 2016 Apr 1. PMID: 27045082.

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Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein

Catalog numberMAGEA1-HB002

HumanE. coliMolecule · MAGE-A1Molecular weight · 35.6 kDa

Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References