Biotinylated Human LILRB4/CD85k/ILT3 Domain 1+hinge Protein

Name: Biotinylated Human LILRB4/CD85k/ILT3 Domain 1+hinge Protein
Brand: Puweituo Bio
SKU: LILRB4-HB004

Catalog numberLILRB4-HB004

HumanHEK293Molecule · HM18Molecular weight · 14.77 kDa

Product introduction

Recombinant Biotinylated Human LILRB4/CD85k/ILT3 Domain 1+hinge Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln22-Lys123 (UniProt accession: Q8NHJ6-1).

Product details

Molecular aliases: HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Protein length: Gln22-Lys123
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 14.77 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human LILRB4 Domain 1+hinge on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human LILRB4 Domain 1+hinge is greater than 90% as determined by SEC-HPLC.

Background

LILRB4,also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.

References

1.Samuel J, Heyu C, Mi D, et al. A Novel Anti-LILRB4 CAR-T Cell for the Treatment of Monocytic AML[J]. Molecular Therapy, 2018:S1525001618303721-.

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Biotinylated Human LILRB4/CD85k/ILT3 Domain 1+hinge Protein

Catalog numberLILRB4-HB004

HumanHEK293Molecule · HM18Molecular weight · 14.77 kDa

Biotinylated Human LILRB4/CD85k/ILT3 Domain 1+hinge Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Human LILRB4/CD85k/ILT3 Domain 1+hinge Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References