Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer Protein

Name: Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer Protein
Brand: Puweituo Bio
SKU: HPV16E6-HP001

Catalog numberHPV16E6-HP001

HumanHEK293Molecule · Human papillomavirus type 16Molecular weight · 50.50 kDa

Product introduction

Recombinant Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KLPQLCTEL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &KLPQLCTEL).

Product details

Molecular aliases: Human papillomavirus type 16; HPV16; E6;
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer is greater than 95% as determined by SEC-HPLC.

Background

Human papillomavirus (HPV) 16 infection is a necessary condition for the pathogenesis and development of cervical cancer. The E6 protein is expressed by the HPV16 E6 gene and promotes malignant phenotype transformation, which is an important mechanism for the occurrence and development of cervical cancer.

References

1.Zhang L, Lai Y, Sun Y, Xu B, Qiang X, Zhou X, Wang T. HPV16 E6 regulates the proliferation, invasion, and apoptosis of cervical cancer cells by downregulating miR-504. Transl Cancer Res. 2020 Dec;9(12):7588-7595. doi: 10.21037/tcr-20-2913. PMID: 35117358; PMCID: PMC8799100.

加载中…

Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer Protein

Catalog numberHPV16E6-HP001

HumanHEK293Molecule · Human papillomavirus type 16Molecular weight · 50.50 kDa

Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References