Biotinylated Human HMGB1 Protein (Primary Amine Labeling)

Name: Biotinylated Human HMGB1 Protein (Primary Amine Labeling)
Brand: Puweituo Bio
SKU: HMGB1-HB001

Catalog numberHMGB1-HB001

HumanHEK293Molecule · HMGB1Molecular weight · 26 kDa

Product introduction

Recombinant Biotinylated Human HMGB1 Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Glu215 (UniProt accession: P09429-1).

Product details

Molecular aliases: HMGB1; HMG1; HMG3; SBP-1; HMG-1; SBP1
Protein length: Met1-Glu215
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 32-36 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per ug by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human HMGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HMGB1 is greater than 95% as determined by SEC-HPLC.

SPR Data — Human AGER, His Tag immobilized on CM5 Chip can bind Biotinylated Human HMGB1, His Tag with an affinity constant of 2.60 μM as determined in SPR assay (Biacore T200).

Background

High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation.

References

1.Yang H, Antoine DJ, Andersson U, Tracey KJ. The many faces of HMGB1: molecular structure-functional activity in inflammation, apoptosis, and chemotaxis. J Leukoc Biol. 2013 Jun;93(6):865-73. doi: 10.1189/jlb.1212662. Epub 2013 Feb 27. PMID: 23446148; PMCID: PMC4051189.

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Biotinylated Human HMGB1 Protein (Primary Amine Labeling)

Catalog numberHMGB1-HB001

HumanHEK293Molecule · HMGB1Molecular weight · 26 kDa

Biotinylated Human HMGB1 Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Background

References

Biotinylated Human HMGB1 Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Background

References