Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein

Name: Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein
Brand: Puweituo Bio
SKU: HLAA1101-HP001

Catalog numberHLAA1101-HP001

HumanE. coliMolecule · RAS11BMolecular weight · 35.36 kDa

Product introduction

Recombinant Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and AVVGASGVGK peptide (accession: AAV53343.1(HLA-A*11:01) & P61769(B2M) &AVVGASGVGK).

Product details

Molecular aliases: RAS11B; AVVGASGVGK; RASL11B
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer is greater than 90% as determined by SEC-HPLC.

Background

RAS (H-ras, K-ras, and N-ras), as the second largest mutated gene driver in various human cancers, has long been a vital research target for cancer. Its function is to transform the extracellular environment into a cascade of intracellular signal transduction. RAS mutant protein regulates tumor cell proliferation, apoptosis, metabolism and angiogenesis through downstream MAPK, PI3K and other signaling pathways.

References

1.Chen S, Li F, Xu D, Hou K, Fang W, Li Y. The Function of RAS Mutation in Cancer and Advances in its Drug Research. Curr Pharm Des. 2019;25(10):1105-1114. doi: 10.2174/1381612825666190506122228. PMID: 31057104.

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Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein

Catalog numberHLAA1101-HP001

HumanE. coliMolecule · RAS11BMolecular weight · 35.36 kDa

Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References