Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer Protein

Name: Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer Protein
Brand: Puweituo Bio
SKU: HLAA0301-HB002

Catalog numberHLAA0301-HB002

HumanE. coliMolecule · Influenza NP; ILRGSVAHKMolecular weight · 35.39 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and ILRGSVAHK peptide (accession: NP_002107.3(HLA-A*03:01) & P61769(B2M) &ILRGSVAHK).

Product details

Molecular aliases: Influenza NP; ILRGSVAHK
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.39 kDa (HLA-A*03:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer is greater than 95% as determined by SEC-HPLC.

Background

Influenza A virus (IAV) is a respiratory pathogen that causes seasonal epidemics with significant mortality. Influenza virus genome encapsidation is essential for the formation of a helical viral ribonucleoprotein (vRNP) complex composed of nucleoproteins (NP), the trimeric polymerase, and the viral genome. The RNA, located at the interface of neighboring NP protomers, interacts with conserved residues previously described as essential for the NP-RNA interaction. The NP undergoes conformational changes to enable RNA binding and helix formation.

References

1.(1) Petrich A, Dunsing V, Bobone S, Chiantia S. Influenza A M2 recruits M1 to the plasma membrane: A fluorescence fluctuation microscopy study. Biophys J. 2021 Dec 21;120(24):5478-5490. doi: 10.1016/j.bpj.2021.11.023. Epub 2021 Nov 19. PMID: 34808098; PMCID: PMC8715234. (2) Chenavier F, Estrozi LF, Teulon JM, Zarkadas E, Freslon LL, Pellequer JL, Ruigrok RWH, Schoehn G, Ballandras-Colas A, Crépin T. Cryo-EM structure of influenza helical nucleocapsid reveals NP-NP and NP-RNA interactions as a model for the genome encapsidation. Sci Adv. 2023 Dec 15;9(50):eadj9974. doi: 10.1126/sciadv.adj9974. Epub 2023 Dec 15. PMID: 38100595; PMCID: PMC10848707.

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Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer Protein

Catalog numberHLAA0301-HB002

HumanE. coliMolecule · Influenza NP; ILRGSVAHKMolecular weight · 35.39 kDa

Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References