Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer Protein

Name: Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer Protein
Brand: Puweituo Bio
SKU: HLAA0301-HB001

Catalog numberHLAA0301-HB001

HumanE. coliMolecule · MHC; IGA2Molecular weight · 35.26 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and FTWTPSSGK peptide (accession: NP_002107.3(HLA-A*03:01) & P61769(B2M) &FTWTPSSGK).

Product details

Molecular aliases: MHC; IGA2
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.26 kDa (HLA-A*03:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA*03:01&B2M&IGA2 (FTWTPSSGK) Monomer, His Tag at 0.5 μg/ml (100 μl/Well) on streptavidin (5 μg/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.0 ng/ml determined by ELISA.

Background

The immunoglobulin variable region is a part of an antibody molecule responsible for antigen recognition and binding. It is located at the N-terminal end of each antibody chain and is highly diverse due to the presence of variable (V), diversity (D), and joining (J) gene segments in B cells that undergo somatic recombination during antibody production. This variability allows the immune system to recognize a wide range of antigens.

References

1.[1] Kang TH, Jung ST. Reprogramming the Constant Region of Immunoglobulin G Subclasses for Enhanced Therapeutic Potency against Cancer. Biomolecules. 2020 Mar 1;10(3):382. doi: 10.3390/biom10030382.
2.[2] Cory S. Immunoglobulin variable region genes. Surv Synth Pathol Res. 1984;3(2):149-64. doi: 10.1159/000156923.

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Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer Protein

Catalog numberHLAA0301-HB001

HumanE. coliMolecule · MHC; IGA2Molecular weight · 35.26 kDa

Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*03:01&B2M&IGA2 (FTWTPSSGK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References