PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein

Name: PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein
Brand: Puweituo Bio
SKU: HLAA0201-HP017

Catalog numberHLAA0201-HP017

HumanHEK293Molecule · MHCMolecular weight · 500 kDa

Product introduction

Recombinant PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidinIt contains Gly25-Thr305 (HLA-A*02:01) and Ile21-Met119 (B2M) (accession: A0A140T913(HLA-A*02:01) & P61769(B2M)).

Product details

Molecular aliases: MHC; HLA-A*02:01; Peptide Ready
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 500 kDa.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 100mM L-arginine, 0.2% BSA (pH 7.4).
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

ELISA Data — Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.

Flow Cytometry Data — 1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (Cat. MHC-HM43RTP) (Loaded PRAME Peptide SLLQHLIGL) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal. (QC Test)

Background

Peptide Ready HLA-A*02:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

References

1.Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
2.[2] Sun Y, Young MC, Woodward CH, Danon JN, Truong H, Gupta S, Winters TJ, Burslem G, Sgourakis NG. Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. bioRxiv [Preprint]. 2023 Mar 18:2023.03.18.533266.

加载中…

PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein

Catalog numberHLAA0201-HP017

HumanHEK293Molecule · MHCMolecular weight · 500 kDa

PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein

Product introduction

Product details

Data display

ELISA

ELISA Data

Flow Cytometry

Flow Cytometry Data

Background

References

PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein

Product introduction

Product details

Data display

ELISA

ELISA Data

Flow Cytometry

Flow Cytometry Data

Background

References