Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer Protein

Name: Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer Protein
Brand: Puweituo Bio
SKU: HLAA0201-HP010

Catalog numberHLAA0201-HP010

HumanE. coliMolecule · TLIRWAILEA; Foxp3Molecular weight · 35.6 kDa

Product introduction

Recombinant Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and TLIRWAILEA peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &TLIRWAILEA).

Product details

Molecular aliases: TLIRWAILEA; Foxp3
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.2ng/ml determined by ELISA.

Background

TLIRWAILEA is position 344-353 of Foxp3 which is a member of a large forkhead family of related proteins. The peptide has minimal homology with other Foxp family members, such as Foxp1, 2 and 4, and it has also been shown to induce strong peptide-specific CD8+ T cell responses, which recognize Foxp3+/HLA-A*02:01+ cutaneous T lymphoma cells.

References

1.[1] WO2017124001A2.
2.[2] Dao T, Mun SS, Scott AC, Jarvis CA, Korontsvit T, Yang Z, Liu L, Klatt MG, Guerreiro M, Selvakumar A, Brea EJ, Oh C, Liu C, Scheinberg DA. Depleting T regulatory cells by targeting intracellular Foxp3 with a TCR mimic antibody. Oncoimmunology. 2019 Apr 15;8(7):1570778. doi: 10.1080/2162402X.2019.1570778. PMID: 31143508; PMCID: PMC6527296.

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Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer Protein

Catalog numberHLAA0201-HP010

HumanE. coliMolecule · TLIRWAILEA; Foxp3Molecular weight · 35.6 kDa

Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References