Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein

Name: Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein
Brand: Puweituo Bio
SKU: HLAA0201-HP008

Catalog numberHLAA0201-HP008

HumanE. coliMolecule · FIFPPSDEQL; IGKC;Molecular weight · 35.6 kDa

Product introduction

Recombinant Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and FIFPPSDEQL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &FIFPPSDEQL).

Product details

Molecular aliases: FIFPPSDEQL; IGKC;
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 18.9 ng/ml determined by ELISA. (QC Test)

Background

IGKC (Immunoglobulin kappa constant) is a segment of the kappa light chain. This region is important for the stability and function of the antibody molecule, as it helps in the proper folding and assembly of the immunoglobulin. Additionally, the kappa constant region may also be involved in the interaction between the antibody and other immune system components.

References

1.[1] Bengtén E, Wilson M, Miller N, Clem LW, Pilström L, Warr GW. Immunoglobulin isotypes: structure, function, and genetics. Curr Top Microbiol Immunol. 2000;248:189-219. doi: 10.1007/978-3-642-59674-2_9. PMID: 10793479.
2.[2] Li M, Feng DY, Ren W, Zheng L, Zheng H, Tang M, Cao Y. Expression of immunoglobulin kappa light chain constant region in abnormal human cervical epithelial cells. Int J Biochem Cell Biol. 2004 Nov;36(11):2250-7. doi: 10.1016/j.biocel.2004.03.017. PMID: 15313470.

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Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein

Catalog numberHLAA0201-HP008

HumanE. coliMolecule · FIFPPSDEQL; IGKC;Molecular weight · 35.6 kDa

Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References