Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer Protein

Name: Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer Protein
Brand: Puweituo Bio
SKU: HLAA0201-HP004

Catalog numberHLAA0201-HP004

HumanE. coliMolecule · AAPGAPAAV; ARFMolecular weight · 35.6 kDa

Product introduction

Recombinant Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and AAPGAPAAV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &AAPGAPAAV).

Product details

Molecular aliases: AAPGAPAAV; ARF
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.7ng/ml determined by ELISA.

Background

Auxin Response Factors (ARFs) are a family of plant-specific transcription factors that play crucial roles in regulating plant growth and development. ARFs bind to auxin response elements (AuxREs) in the promoters of auxin-responsive genes, where they activate or repress transcription. They also recruit a second family of transcription factors, the Aux/IAA repressors, which confer an auxin response to the genes.

References

1.[1] Jing H, Korasick DA, Emenecker RJ, Morffy N, Wilkinson EG, Powers SK, Strader LC. Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning. Nat Commun. 2022 Jul 11;13(1):4015. doi: 10.1038/s41467-022-31628-2.
2.[2] Li SB, Xie ZZ, Hu CG, Zhang JZ. A Review of Auxin Response Factors (ARFs) in Plants. Front Plant Sci. 2016 Feb 3;7:47. doi: 10.3389/fpls.2016.00047.

加载中…

Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer Protein

Catalog numberHLAA0201-HP004

HumanE. coliMolecule · AAPGAPAAV; ARFMolecular weight · 35.6 kDa

Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Human HLA-A*02:01&B2M&ARF (AAPGAPAAV) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References