Biotinylated Human GPRC5D Nanodisc

Name: Biotinylated Human GPRC5D Nanodisc
Brand: Puweituo Bio
SKU: GPRC5D-HB004

Catalog numberGPRC5D-HB004

HumanHEK293Molecule · GPRC5DMolecular weight · 51.70 kDa

Product introduction

Recombinant Biotinylated Human GPRC5D Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val345 (UniProt accession: Q9NZD1-1).

Product details

Molecular aliases: GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor
Protein length: Met1-Val345
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 51.70 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).

BLI Data — Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-GPRC5D Antibody, hFc Tag with an affinity constant of 2.21 nM as determined in BLI assay.

Background

G protein–coupled receptor, family C, group 5, member D (GPRC5D) is an orphan receptor that is primarily expressed in plasma cells and hard keratinized tissues, with low expression in normal human tissues. GPRC5D expression is enriched in malignant plasma cells and is associated with markers of high-risk myeloma.

References

1.Chari A, Minnema MC, Berdeja JG, Oriol A, van de Donk NWCJ, Rodríguez-Otero P, Askari E, Mateos MV, Costa LJ, Caers J, Verona R, Girgis S, Yang S, Goldsmith RB, Yao X, Pillarisetti K, Hilder BW, Russell J, Goldberg JD, Krishnan A. Talquetamab, a T-Cell-Redirecting GPRC5D Bispecific Antibody for Multiple Myeloma. N Engl J Med. 2022 Dec 15;387(24):2232-2244. doi: 10.1056/NEJMoa2204591. Epub 2022 Dec 10. PMID: 36507686.

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Biotinylated Human GPRC5D Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

BLI

BLI Data

Background

References

Biotinylated Human GPRC5D Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

BLI

BLI Data

Background

References