Biotinylated Human GPRC5D VLP

Name: Biotinylated Human GPRC5D VLP
Brand: Puweituo Bio
SKU: GPRC5D-HB003

Catalog numberGPRC5D-HB003

HumanHEK293Molecule · GPRC5DMolecular weight · 39.6 kDa

Product introduction

Recombinant Biotinylated Human GPRC5D VLP is expressed in HEK293 cells. It contains amino acid residues Met1-Val345 (It may have cross reaction with anti-His antibody) (UniProt accession: Q9NZD1-1).

Product details

Molecular aliases: GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor
Protein length: Met1-Val345
Expression system: HEK293
Molecular weight: The target protein has a predicted MW of 39.6 kDa.
Purity: > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22μm filtered solution in PBS, 300mM L-arginine (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: GPR-HM05CB

Data display

SEC-HPLC Data — The purity of Biotinylated Human GPRC5D VLP is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human GPRC5D VLP at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).

SPR Data — Biotinylated Human GPRC5D VLP captured on CM5 Chip via Streptavidin can bind Anti-GPRC5D Antibody with an affinity constant of 0.28 nM as determined in SPR assay (Biacore T200).

Background

G protein–coupled receptor, family C, group 5, member D (GPRC5D) is an orphan receptor that is primarily expressed in plasma cells and hard keratinized tissues, with low expression in normal human tissues. GPRC5D expression is enriched in malignant plasma cells and is associated with markers of high-risk myeloma.

References

1.Chari A, Minnema MC, Berdeja JG, Oriol A, van de Donk NWCJ, Rodríguez-Otero P, Askari E, Mateos MV, Costa LJ, Caers J, Verona R, Girgis S, Yang S, Goldsmith RB, Yao X, Pillarisetti K, Hilder BW, Russell J, Goldberg JD, Krishnan A. Talquetamab, a T-Cell-Redirecting GPRC5D Bispecific Antibody for Multiple Myeloma. N Engl J Med. 2022 Dec 15;387(24):2232-2244. doi: 10.1056/NEJMoa2204591. Epub 2022 Dec 10. PMID: 36507686.

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Biotinylated Human GPRC5D VLP

Catalog numberGPRC5D-HB003

HumanHEK293Molecule · GPRC5DMolecular weight · 39.6 kDa

Product introduction

Recombinant Biotinylated Human GPRC5D VLP is expressed in HEK293 cells. It contains amino acid residues Met1-Val345 (It may have cross reaction with anti-His antibody) (UniProt accession: Q9NZD1-1).

Biotinylated Human GPRC5D VLP

Product introduction

Product details

Data display

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human GPRC5D VLP

Product introduction

Product details

Data display

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References