Human FGF1 Protein

Name: Human FGF1 Protein
Brand: Puweituo Bio
SKU: FGF1-HP001

Catalog numberFGF1-HP001

HumanE. coliMolecule · FGF-1Molecular weight · 15.83 kDa

Product introduction

Recombinant Human FGF1 Protein is expressed in E. coli without tag. It contains amino acid residues Phe16-Asp155 (UniProt accession: P05230-1).

Product details

Molecular aliases: FGF-1; aFGF; ECGF; HBGF-1; FGFA; FGF1; HBGF1; Fibroblast growth factor-1; Fibroblast growth factor 1
Protein length: Phe16-Asp155
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 15.83 kDa same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human FGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human FGF1 is greater than 95% as determined by SEC-HPLC.

SPR Data — Human FGFR1 alpha (IIIc), His Tag captured on CM5 Chip via Anti-His Antibody can bind Human FGF1, No tag with an affinity constant of 42.45 nM as determined in SPR assay (Biacore T200).

Cell Proliferation Bioactivity — Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.7 ng/mL (QC Test).

Background

Fibroblast Growth Factor 1 (Fgf1), also known as acidic FGF (aFGF), is involved in the regulation of various biological processes, ranging from development to disease pathogenesis. It is a single chain polypeptide and is highly expressed in adult brain and kidney tissues. Its expression has been shown to be directed by multiple tissue-specific promoters, which generate transcripts of varying lengths.

References

1.Jamal SB, Hockman D. FGF1. Differentiation. 2024 Sep-Oct;139:100802. doi: 10.1016/j.diff.2024.100802. Epub 2024 Jul 22. PMID: 39074995.

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Human FGF1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Bioactivity

Cell Proliferation Bioactivity

Background

References

Human FGF1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Bioactivity

Cell Proliferation Bioactivity

Background

References