Human EGF Protein

Name: Human EGF Protein
Brand: Puweituo Bio
SKU: EGF-HP001

Catalog numberEGF-HP001

HumanE. coliMolecule · HOMG4; EGF; URGMolecular weight · 5.6 kDa

Product introduction

Recombinant Human EGF Protein is expressed in E. coli without tag. It contains amino acid residues Asn971-Arg1023 (UniProt accession: P01133-1).

Product details

Molecular aliases: HOMG4; EGF; URG
Protein length: Asn971-Arg1023
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 5.6 kDa same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human EGF is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Human EGF, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.55μg/ml determined by ELISA. (QC Test)

SPR Data — Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).

Cell Proliferation Bioactivity — Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02 - 0.2 ng/mL (QC Test).

Background

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

References

1.Normanno N, Bianco C, De Luca A, Salomon DS. The role of EGF-related peptides in tumor growth. Front Biosci. 2001 May 1;6:D685-707. doi: 10.2741/normano. PMID: 11333208.

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Human EGF Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Proliferation Bioactivity

Background

References

Human EGF Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Proliferation Bioactivity

Background

References