Biotinylated Human DKK1 Protein

Name: Biotinylated Human DKK1 Protein
Brand: Puweituo Bio
SKU: DKK1-HB002

Catalog numberDKK1-HB002

HumanHEK293Molecule · Dickkopf-1; Dkk1; SKMolecular weight · 28.5 kDa

Product introduction

Recombinant Biotinylated Human DKK1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr32-His266 (UniProt accession: O94907).

Product details

Molecular aliases: Dickkopf-1; Dkk1; SK
Protein length: Thr32-His266
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human DKK1 is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human DKK1, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA (QC Test).

Background

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.

References

1.Zhang W, Drake M T. Potential Role for Therapies Targeting DKK1, LRP5, and Serotonin in the Treatment of Osteoporosis[J]. Current Osteoporosis Reports, 2012, 10(1):93-100.

加载中…

Biotinylated Human DKK1 Protein

Catalog numberDKK1-HB002

HumanHEK293Molecule · Dickkopf-1; Dkk1; SKMolecular weight · 28.5 kDa

Product introduction

Recombinant Biotinylated Human DKK1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr32-His266 (UniProt accession: O94907).

Biotinylated Human DKK1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

Background

References

Biotinylated Human DKK1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

Background

References