Biotinylated Human CLEC9A Protein (Primary Amine Labeling)

Name: Biotinylated Human CLEC9A Protein (Primary Amine Labeling)
Brand: Puweituo Bio
SKU: CLEC9A-HF002

Catalog numberCLEC9A-HF002

HumanHEK293Molecule · CD370Molecular weight · 48.5 kDa

Product introduction

Recombinant Biotinylated Human CLEC9A Protein (Primary Amine Labeling) is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Lys57-Val241 (UniProt accession: Q6UXN8).

Product details

Molecular aliases: CD370; CLEC9A; 9830005G06Rik; DNGR-1; HEEE9341; UNQ9341;DNGR1
Protein length: Lys57-Val241
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human CLEC9A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human CLEC9A is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 30.9ng/ml determined by ELISA (QC Test).

Background

CLEC9A is a recently discovered C-type lectin receptor involved in sensing necrotic cells. In humans, this receptor is selectively expressed by BDCA3(+) myeloid dendritic cells (mDCs), which have been proposed to be the main human cross-presenting mDCs and may represent the human homologue of murine CD8(+) DCs. In mice, it was demonstrated that antigens delivered with antibodies to CLEC9A are presented by CD8(+) DCs to both CD4(+) and CD8(+) T cells and induce antitumor immunity in a melanoma model.

References

1.Schreibelt G, Klinkenberg LJ, Cruz LJ, Tacken PJ, Tel J, Kreutz M, Adema GJ, Brown GD, Figdor CG, de Vries IJ. The C-type lectin receptor CLEC9A mediates antigen uptake and (cross-)presentation by human blood BDCA3+ myeloid dendritic cells. Blood. 2012 Mar 8;119(10):2284-92. doi: 10.1182/blood-2011-08-373944. Epub 2012 Jan
2.PMID:
3.Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Biotinylated Human CLEC9A Protein (Primary Amine Labeling)

Catalog numberCLEC9A-HF002

HumanHEK293Molecule · CD370Molecular weight · 48.5 kDa

Biotinylated Human CLEC9A Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human CLEC9A Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References