Human B7-1/CD80 Protein

Name: Human B7-1/CD80 Protein
Brand: Puweituo Bio
SKU: CD80-HF001

Catalog numberCD80-HF001

HumanHEK293Molecule · CD80 moleculeMolecular weight · 50.5 kDa

Product introduction

Recombinant Human B7-1/CD80 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Val35-Asn242 (UniProt accession: P33681-1).

Product details

Molecular aliases: CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7
Protein length: Val35-Asn242
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human B7-1 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human CTLA-4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. (QC Test)

SPR Data — Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag (Cat. CD8-HM128) with an affinity constant of 8.77 μM as determined in SPR assay (Biacore S200).

Background

Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.

References

1.Merwe V D, P. A. CD80 (B7-1) Binds Both CD28 and CTLA-4 with a Low Affinity and Very Fast Kinetics[J]. Journal of Experimental Medicine, 1997, 185(3):393-404.D Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

加载中…

Human B7-1/CD80 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Human B7-1/CD80 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References