Biotinylated Human CD73/NT5E Protein

Name: Biotinylated Human CD73/NT5E Protein
Brand: Puweituo Bio
SKU: CD73-HB001

Catalog numberCD73-HB001

HumanHEK293Molecule · CD73Molecular weight · 60.92 kDa

Product introduction

Recombinant Biotinylated Human CD73/NT5E Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Trp27-Lys547 (UniProt accession: P21589-1).

Product details

Molecular aliases: CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA;5NT; EC3.1.3.5
Protein length: Trp27-Lys547
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 60.92 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human CD73 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human CD73, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.

Background

CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.

References

1.H? usler SF, Del Barrio I M, Diessner J, et al. Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion.[J]. Am J Transl Res, 2014, 6(2):129-139.

加载中…

Biotinylated Human CD73/NT5E Protein

Catalog numberCD73-HB001

HumanHEK293Molecule · CD73Molecular weight · 60.92 kDa

Product introduction

Recombinant Biotinylated Human CD73/NT5E Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Trp27-Lys547 (UniProt accession: P21589-1).

Biotinylated Human CD73/NT5E Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human CD73/NT5E Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References