Mouse CD40 Ligand/TNFSF5 Trimer Protein

Name: Mouse CD40 Ligand/TNFSF5 Trimer Protein
Brand: Puweituo Bio
SKU: CD40-MF001

Catalog numberCD40-MF001

MouseHEK293Molecule · CD40LMolecular weight · 75.6 kDa

Product introduction

Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed in HEK293 cells with monomeric hFc tag at the N-terminus. It contains amino acid residues Met112-Leu260 trimer design (UniProt accession: P27548).

Product details

Molecular aliases: CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Protein length: Met112-Leu260
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Mouse CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Mouse CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Mouse CD40, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA. (QC Test)

BLI Data — Loaded Mouse CD40, His Tag on Anti-His-Biosensor can bind Mouse CD40 Ligand (Trimer), monomeric hFc Tag with an affinity constant of 0.10 nM as determined in BLI assay.

Background

CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

References

1.Pound, J. D. Minimal cross-linking and epitope requirements for CD40-dependent suppression of apoptosis contrast with those for promotion of the cell cycle and homotypic adhesions in human B cells[J]. International Immunology, 1999, 11(1):11-20 Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Mouse CD40 Ligand/TNFSF5 Trimer Protein

Catalog numberCD40-MF001

MouseHEK293Molecule · CD40LMolecular weight · 75.6 kDa

Mouse CD40 Ligand/TNFSF5 Trimer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

Background

References

Mouse CD40 Ligand/TNFSF5 Trimer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

Background

References