Biotinylated Human Siglec-5/CD170 Protein

Name: Biotinylated Human Siglec-5/CD170 Protein
Brand: Puweituo Bio
SKU: CD170-HB001

Catalog numberCD170-HB001

HumanHEK293Molecule · CD170Molecular weight · 49.3 kDa

Product introduction

Recombinant Biotinylated Human Siglec-5/CD170 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu17-Thr434 (UniProt accession: O15389).

Product details

Molecular aliases: CD170; CD33 antigen-like 2; CD33L2; OBBP2; Siglec5; SIGLEC5; OB-BP2
Protein length: Glu17-Thr434
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 49.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Purity: > 90% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS, 350mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human Siglec-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

SEC-HPLC Data — The purity of Biotinylated Human Siglec-5 is greater than 90% as determined by SEC-HPLC.

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Siglec 5 is putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

References

1.Macauley M S, Crocker P R, Paulson J C. Siglec-mediated regulation of immune cell function in disease[J]. Nature Reviews Immunology, 2014, 14(10):653-666.

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Biotinylated Human Siglec-5/CD170 Protein

Catalog numberCD170-HB001

HumanHEK293Molecule · CD170Molecular weight · 49.3 kDa

Biotinylated Human Siglec-5/CD170 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Human Siglec-5/CD170 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References