Human M-CSF R/CSF1R/CD115 Protein

Name: Human M-CSF R/CSF1R/CD115 Protein
Brand: Puweituo Bio
SKU: CD115-HF001

Catalog numberCD115-HF001

HumanHEK293Molecule · CD115Molecular weight · 81.3 kDa

Product introduction

Recombinant Human M-CSF R/CSF1R/CD115 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ile20-Glu512 (UniProt accession: P07333-1).

Product details

Molecular aliases: CD115; CSF1R; CSF-1-R; CSFR; FMSFIM2; M-CSF R; MCSFR; M-CSFR; C-FMS; FIM2; FMS; HDLS
Protein length: Ile20-Glu512
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 81.3 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human M-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human M-CSF R is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human M-CSF, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA (QC Test).

SPR Data — Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).

Background

CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region. CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

References

1.Cannarile M A, Weisser M, Jacob W, et al. Colony-stimulating factor 1 receptor (CSF1R) inhibitors in cancer therapy[J]. Journal for ImmunoTherapy of Cancer, 2017, 5(1):53.

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Human M-CSF R/CSF1R/CD115 Protein

Catalog numberCD115-HF001

HumanHEK293Molecule · CD115Molecular weight · 81.3 kDa

Product introduction

Recombinant Human M-CSF R/CSF1R/CD115 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ile20-Glu512 (UniProt accession: P07333-1).

Human M-CSF R/CSF1R/CD115 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Human M-CSF R/CSF1R/CD115 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References