Biotinylated Human BTLA Protein

Name: Biotinylated Human BTLA Protein
Brand: Puweituo Bio
SKU: BTLA-HB003

Catalog numberBTLA-HB003

HumanHEK293Molecule · CD272Molecular weight · 16.7 kDa

Product introduction

Recombinant Biotinylated Human BTLA Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Lys31-Ser150 (UniProt accession: Q7Z6A9-1).

Product details

Molecular aliases: CD272; BTLA; BTLA1; FLJ16065; MGC129743
Protein length: Lys31-Ser150
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human BTLA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human BTLA is greater than 95% as determined by SEC-HPLC

ELISA Data — Immobilized Biotinylated Human BTLA, His Tag at 2μg/ml(100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human HVEM, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).

Background

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.

References

1.Haymaker C L, Wu R C, Ritthipichai K, et al. BTLA marks a less-differentiated tumor-infiltrating lymphocyte subset in melanoma with enhanced survival properties[J]. OncoImmunology, 2015, 4(8):e1014246. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Biotinylated Human BTLA Protein

Catalog numberBTLA-HB003

HumanHEK293Molecule · CD272Molecular weight · 16.7 kDa

Product introduction

Recombinant Biotinylated Human BTLA Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Lys31-Ser150 (UniProt accession: Q7Z6A9-1).

Biotinylated Human BTLA Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

Background

References

Biotinylated Human BTLA Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

Background

References