Biotinylated Human BDCA-2 Protein

Name: Biotinylated Human BDCA-2 Protein
Brand: Puweituo Bio
SKU: BDCA2-HB001

Catalog numberBDCA2-HB001

HumanHEK293Molecule · BDCA-2Molecular weight · 22.7 kDa

Product introduction

Recombinant Biotinylated Human BDCA-2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Phe46-Ile213 (UniProt accession: Q8WTT0-1).

Product details

Molecular aliases: BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303;
Protein length: Phe46-Ile213
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 22.7 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Bis-Tris PAGE result.
Purity: > 90% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22μm filtered solution in 20mM Tris, 300mM NaCl, 300mM L-arginine (pH 8.5).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

ELISA Data — Immobilized Biotinylated Human BDCA-2, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. (QC Test)

Background

BDCA-2 is a novel type II C-type lectin specifically expressed by plasmacytoid dendritic cells (PDCs) that can internalize antigen for presentation to T cells. Furthermore, signaling via BDCA-2 may play a role in switching from interferon (IFN)-α/β-controlled to interleukin (IL)-12-controlled immune response pathways, as triggering of BDCA-2 potently inhibits secretion of IFN-α/β by PDCs and thereby promotes IL-12 p70 production in PDCs and other cells.

References

1.Jégouzo SA, Feinberg H, Dungarwalla T, Drickamer K, Weis WI, Taylor ME. A Novel Mechanism for Binding of Galactose-terminated Glycans by the C-type Carbohydrate Recognition Domain in Blood Dendritic Cell Antigen 2. J Biol Chem. 2015 Jul 3;290(27):16759-71. doi: 10.1074/jbc. M115.660613. Epub 2015 May 20. PMID: 25995448; PMCID: PMC4505424.

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Biotinylated Human BDCA-2 Protein

Catalog numberBDCA2-HB001

HumanHEK293Molecule · BDCA-2Molecular weight · 22.7 kDa

Product introduction

Recombinant Biotinylated Human BDCA-2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Phe46-Ile213 (UniProt accession: Q8WTT0-1).

Biotinylated Human BDCA-2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References

Biotinylated Human BDCA-2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References