Human BDCA-2 Protein

Name: Human BDCA-2 Protein
Brand: Puweituo Bio
SKU: BDCA2-HF001

Catalog numberBDCA2-HF001

HumanHEK293Molecule · BDCA-2Molecular weight · 47.1 kDa

Product introduction

Recombinant Human BDCA-2 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Phe46-Ile213 (UniProt accession: Q8WTT0-1).

Product details

Molecular aliases: BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303;
Protein length: Phe46-Ile213
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 47.1 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Human BDCA-2, hFc Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 31.8 ng/ml determined by ELISA (QC Test).

Background

BDCA-2 is a novel type II C-type lectin specifically expressed by plasmacytoid dendritic cells (PDCs) that can internalize antigen for presentation to T cells. Furthermore, signaling via BDCA-2 may play a role in switching from interferon (IFN)-α/β-controlled to interleukin (IL)-12-controlled immune response pathways, as triggering of BDCA-2 potently inhibits secretion of IFN-α/β by PDCs and thereby promotes IL-12 p70 production in PDCs and other cells.

References

1.Jégouzo SA, Feinberg H, Dungarwalla T, Drickamer K, Weis WI, Taylor ME. A Novel Mechanism for Binding of Galactose-terminated Glycans by the C-type Carbohydrate Recognition Domain in Blood Dendritic Cell Antigen 2. J Biol Chem. 2015 Jul 3;290(27):16759-71. doi: 10.1074/jbc.M115.660613. Epub 2015 May 20. PMID: 25995448; PMCID: PMC4505424.

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Human BDCA-2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References

Human BDCA-2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References