Biotinylated Human/Mouse/Rat Activin A Protein

Name: Biotinylated Human/Mouse/Rat Activin A Protein
Brand: Puweituo Bio
SKU: ACTIVINA-HB001

Catalog numberACTIVINA-HB001

HumanHEK293Molecule · INHBA; Activin A;Molecular weight · 14.78 kDa

Product introduction

Recombinant Biotinylated Human/Mouse/Rat Activin A Protein is expressed in HEK293 cells with an Avi tag at the C-terminus. It contains amino acid residues Gly311-Ser426 (UniProt accession: P08476).

Product details

Molecular aliases: INHBA; Activin A;
Protein length: Gly311-Ser426
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 14.78 kDa. Due to glycosylation, the protein migrates to 15-18 kDa under reduced (R) condition, and 23-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 4mM HCL.
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

ELISA Data — Immobilized Human/Cynomolgus Activin RIIB, His Tag (Cat. ARB-HM12B) at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi tag with the EC50 of 3.2 ng/ml determined by ELISA. (QC Test)

BLI Data — Loaded Biotinylated Human Activin A, Avi tag on Streptavidin-Biosensor can bind Human Activin RIIB, His Tag with an affinity constant of 30.40 nM as determined in BLI assay.

Blocking Data — Serial dilutions of Bimagrumab were added into Human/Cynomolgus Activin RIIB, His Tag: Biotinylated Human/Mouse/Rat Activin A, Avi tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14μg/ml.

Background

Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.

References

1.Morianos I, Papadopoulou G, Semitekolou M, Xanthou G. Activin-A in the regulation of immunity in health and disease. J Autoimmun. 2019 Nov;104:102314. doi: 10.1016/j.jaut.2019.102314. Epub 2019 Aug 13. PMID: 31416681.

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Biotinylated Human/Mouse/Rat Activin A Protein

Catalog numberACTIVINA-HB001

HumanHEK293Molecule · INHBA; Activin A;Molecular weight · 14.78 kDa

Product introduction

Recombinant Biotinylated Human/Mouse/Rat Activin A Protein is expressed in HEK293 cells with an Avi tag at the C-terminus. It contains amino acid residues Gly311-Ser426 (UniProt accession: P08476).

Biotinylated Human/Mouse/Rat Activin A Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

ELISA

ELISA Data

BLI

BLI Data

Blocking

Blocking Data

Background

References

Biotinylated Human/Mouse/Rat Activin A Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

ELISA

ELISA Data

BLI

BLI Data

Blocking

Blocking Data

Background

References