Human EpCAM/TROP1 Protein

Name: Human EpCAM/TROP1 Protein
Brand: Puweituo Bio
SKU: TROP1-HF001

Catalog numberTROP1-HF001

HumanHEK293Molecule · CD326Molecular weight · 54.2 kDa

Product introduction

Recombinant Human EpCAM/TROP1 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln24-Lys265 (UniProt accession: P16422).

Product details

Molecular aliases: CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; ACSTD1; DIAR5; gp40; HNPCC8; MOC31; TACST-1; TACSTD1; TROP1; ESA; KSA; MIC18; MK1;EGP2; TACST1
Protein length: Gln24-Lys265
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..

SEC-HPLC Data — The purity of Human EpCAM is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human EpCAM, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test).

SPR Data — Human EpCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human AGR-2, His Tag with an affinity constant of 6.91 μM as determined in SPR assay (Biacore T200).

Background

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.

References

1.Pak M G, Shin D H, Lee C H, et al. Significance of EpCAM and TROP2 expression in non-small cell lung cancer[J]. World Journal of Surgical Oncology, 2012, 10(1):53 Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Human EpCAM/TROP1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Human EpCAM/TROP1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References