Non-biotinylated Mouse TNFSF15 Protein (active trimer)

Name: Non-biotinylated Mouse TNFSF15 Protein (active trimer)
Brand: Puweituo Bio
SKU: TNFSF15-MP001

Catalog numberTNFSF15-MP001

MouseHEK293Molecule · TL1AMolecular weight · 24.40 kDa

Product introduction

Recombinant Mouse TNFSF15 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Ala61-Leu252 (UniProt accession: Q5UBV8).

Product details

Molecular aliases: TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A; VEGI251
Protein length: Ala61-Leu252
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 24.40 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Mouse TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Mouse TNFSF15 is greater than 90% as determined by SEC-HPLC.

SEC-MALS Data — The purity of Mouse TNFSF15 is greater than 90% and the molecular weight of this protein is around 70-95 kDa as determined by SEC-MALS.

ELISA Data — Immobilized Mouse TNFSF15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).

SPR Data — Mouse DR3, His Tag immobilized on CM5 Chip can bind Mouse TNFSF15, His Tag with an affinity constant of 0.96 nM as determined in SPR assay (Biacore T200).

Background

TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins. TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation. Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation.

References

1.Valatas V, Kolios G, Bamias G. TL1A (TNFSF15) and DR3 (TNFRSF25): A Co-stimulatory System of Cytokines With Diverse Functions in Gut Mucosal Immunity. Front Immunol. 2019 Mar 27;10:583. doi: 10.3389/fimmu.2019.00583. PMID: 30972074; PMCID: PMC6445966.

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Non-biotinylated Mouse TNFSF15 Protein (active trimer)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Non-biotinylated Mouse TNFSF15 Protein (active trimer)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References