Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer Protein

Name: Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer Protein
Brand: Puweituo Bio
SKU: TERT-HB002

Catalog numberTERT-HB002

HumanE. coliMolecule · TERT; ILAKFLHWLMolecular weight · 35.36 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGLAK peptide (accession: AAV53343.1(HLA-A*11:01) & P61769(B2M) &KITDFGLAK).

Product details

Molecular aliases: TERT; ILAKFLHWL
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer, His Avi tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for HLA-A*11:01&B2M&EGFR TCR, hFc Tag with the EC50 of 32.6ng/ml determined by ELISA. (QC Test)

Background

The human telomerase catalytic subunit human telomerase reverse transcriptase (hTERT) is expressed in many human cancer cells derived from multiple tissues, but infrequently in normal cells. Thus, hTERT is an attractive candidate target for tumor immunotherapy. hTERT:540–548 peptide (p540, ILAKFLHWL) has been identified as an effective HLA-A*0201-restricted T-cell epitope, an effective T-cell-based cancer treatment, including vaccines, against hTERT will likely require the identification of other MHC class I-restricted epitopes in this antigen.

References

1.Huang G, Geng J, Wang R, Chen L. Identification of a new cytotoxic T-cell epitope p675 of human telomerase reverse transcriptase. Cancer Biother Radiopharm. 2012 Nov;27(9):600-5. doi: 10.1089/cbr.2012.1193. Epub 2012 Aug 23. PMID: 22917214.

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Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer Protein

Catalog numberTERT-HB002

HumanE. coliMolecule · TERT; ILAKFLHWLMolecular weight · 35.36 kDa

Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References