Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer Protein

Name: Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer Protein
Brand: Puweituo Bio
SKU: TDT-HP001

Catalog numberTDT-HP001

HumanHEK293Molecule · DNA nucleotidylexotransferaseMolecular weight · 50.50 kDa

Product introduction

Recombinant Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYDKTKRI peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &ALYDKTKRI).

Product details

Molecular aliases: DNA nucleotidylexotransferase; terminal deoxynucleotidyl transferase
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.3ng/ml determined by ELISA.

Background

The terminal deoxynucleotidyl transferase (TdT) belongs to the X family of DNA polymerases. This unusual polymerase catalyzes the template-independent addition of random nucleotides on 3'-overhangs during V(D)J recombination. The biological function and intrinsic biochemical properties of the TdT have spurred the development of numerous oligonucleotide-based tools and methods, especially if combined with modified nucleoside triphosphates.

References

1.Sarac I, Hollenstein M. Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids. Chembiochem. 2019 Apr 1;20(7):860-871. doi: 10.1002/cbic.201800658. Epub 2019 Jan 25. PMID: 30451377.

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Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer Protein

Catalog numberTDT-HP001

HumanHEK293Molecule · DNA nucleotidylexotransferaseMolecular weight · 50.50 kDa

Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References