Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Tetramer Protein

Name: Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Tetramer Protein
Brand: Puweituo Bio
SKU: SURVIVIN2B-HP003

Catalog numberSURVIVIN2B-HP003

HumanHEK293Molecule · svn-2BMolecular weight · 258 kDa

Product introduction

Recombinant Human Survivin 2B(HLA-A*24:02) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and AYACNTSTL peptide (accession: AAA59600.1(HLA-A*24:02) & P61769(B2M) &AYACNTSTL).

Product details

Molecular aliases: svn-2B; Survivin-2B; svn 2B
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Tetramer is greater than 95% as determined by SEC-HPLC.

Background

Survivin-2B, a known splice variant of survivin, has been reported to promote cell death in some cancer cells, although it keeps prosurvival function in others.survivin-2B promoted autophagy and further regulated cell death by accumulating and stabilizing IKK alpha in the nucleus.

References

1.Shi K, An J, Shan L, Jiang Q, Li F, Ci Y, Wu P, Duan J, Hui K, Yang Y, Xu C. Survivin-2B promotes autophagy by accumulating IKK alpha in the nucleus of selenite-treated NB4 cells. Cell Death Dis. 2014 Feb 20;5(2):e1071. doi: 10.1038/cddis.2014.34. PMID: 24556686; PMCID: PMC3944251.

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Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Tetramer Protein

Catalog numberSURVIVIN2B-HP003

HumanHEK293Molecule · svn-2BMolecular weight · 258 kDa

Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Tetramer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Tetramer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References