Biotinylated Human SKP1 Protein

Name: Biotinylated Human SKP1 Protein
Brand: Puweituo Bio
SKU: SKP1-HB001

Catalog numberSKP1-HB001

HumanHEK293Molecule · p19AMolecular weight · 21.43 kDa

Product introduction

Recombinant Biotinylated Human SKP1 Protein is expressed in HEK293 cells His tag at the N-terminus and Avi tag at the C-terminusIt contains Pro2-Lys163 (UniProt accession: P63208-1).

Product details

Molecular aliases: p19A; OCP-2; OCP-II; SIII; EMC19; OCP2; SKP1A; TCEB1L
Protein length: Pro2-Lys163
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 21.43 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human SKP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human SKP1 is greater than 95% as determined by SEC-HPLC.

Background

The S-phase Kinase-Associated Protein 1 (SKP1) is a core component of the SKP1, Cullin 1, F-box protein (SCF) complex, an E3 ubiquitin ligase that serves to poly-ubiquitinate a vast array of protein targets as a signal for their proteasomal degradation, thereby playing a critical role in the regulation of downstream biological processes.

References

1.Thompson LL, Rutherford KA, Lepage CC, McManus KJ. Aberrant SKP1 Expression: Diverse Mechanisms Impacting Genome and Chromosome Stability. Front Cell Dev Biol. 2022 Mar 8;10:859582. doi: 10.3389/fcell.2022.859582. PMID: 35345853; PMCID: PMC8957228.

加载中…

Biotinylated Human SKP1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Human SKP1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References