Human RANKL/TNFSF11/CD254 Protein

Name: Human RANKL/TNFSF11/CD254 Protein
Brand: Puweituo Bio
SKU: RANKL-HP001

Catalog numberRANKL-HP001

HumanHEK293Molecule · ODFMolecular weight · 20.5 kDa

Product introduction

Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed in HEK293 cells without tag. It contains amino acid residues Gly63-Asp244 (UniProt accession: O14788-2).

Product details

Molecular aliases: ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2
Protein length: Gly63-Asp244
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Bis-Tris PAGE resu
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human RANKL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human RANKL is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human RANKL, No Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test).

SPR Data — Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).

Background

Receptor activator of nuclear factor κB (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.

References

1.Nagy V, Penninger JM. The RANKL-RANK Story. Gerontology. 2015;61(6):534-42. doi: 10.1159/000371845. Epub 2015 Feb
2.PMID: 25720990.

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Human RANKL/TNFSF11/CD254 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Human RANKL/TNFSF11/CD254 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References