Synonym
PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
Source / expression
Recombinant Human PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe177) and N-Terminus fragment (Ser21-Lys155) [Accession | P00749-1].
Molecular weight
The protein has a predicted MW of 47.5 kDa. The protein migrates to 30-38 kDa and 17 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22 μm filtered solution in 1% HCOOH, 1 mM DTT (pH 3.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 1% HCOOH (pH 3.0). Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
