Synonym
Apo-2L; TRAIL; CD253; TNFSF10; Apo-2 ligand; APO2L; TL2
Source / expression
Recombinant Human TRAIL/TNFSF10 Protein is expressed from E.coli without tag.It contains Val114-Gly281 [Accession | NP_003801.1].
Molecular weight
The protein has a predicted MW of 19.6 kDa same as Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22 μm filtered solution in 50mM Tris, 300mM NaCl (pH 7.2). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
