Synonym
CD329; CDw329; FOAP-9; OBBP-Like; Siglec-9; SIGLEC9
Source / expression
Recombinant human Siglec-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Gly348 [Accession | Q9Y336-1].
Molecular weight
The protein has a predicted MW of 38.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 90% as determined by HPLC
Formulation
Lyophilized from 0.22 μm filtered solution in 25mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 25mM MES (pH 5.5). Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
