Synonym
PC9; PCSK9; FH3; HCHOLA3; LDLCQ1; NARC-1
Source / expression
Recombinant Human PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln31-Gln692 [Accession | Q8NBP7-1].
Molecular weight
Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Supplied as 0.22μm filtered solution in 20mM PB, 300mM NaCl, 10% Glycerol (pH 7.4).
Storage
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
