Synonym
CD158Z; KIR2DS2; KIR3DL7; KIR44; KIRC1; KIR3DL3
Source / expression
Recombinant Human KIR3DL3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu26-Leu322 [Accession | Q8N743].
Molecular weight
The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 0.2M L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
