Synonym
Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE
Source / expression
Recombinant Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile67-Ser293 [Accession | Q9Y337].
Molecular weight
The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 42-48 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 50mM MES (pH 5.5). Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
