Synonym
CSF3; GCSF; C17orf33; CSF3OS;
Source / expression
Recombinant Human G-CSF Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Thr31-Pro204 [Accession | NP_757373.1].
Molecular weight
The protein has a predicted MW of 44.20 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
