Synonym
Ephrin-A3; Ephrin A3; EFL-2; EHK1 ligand; EHK1-L; EFNA3; EPLG3; LERK3
Source / expression
Recombinant Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Ser213 [Accession | P52797-1(Human) / A0A2K5VZR7(Cynomolgus) / NP_001252612.1(Rhesus macaque)].
Molecular weight
The protein has a predicted MW of 22.51 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
