Synonym
EC:3.4.21.45; C3B/C4B inactivator; CFI; IF; C3BINA; FIC3b-INA; I factor (complement); KAF
Source / expression
Recombinant Human Complement Factor I Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys19-Val583 [Accession | P05156].
Molecular weight
The protein has a predicted MW of 64.58 kDa. Due to autocatalytic cleavage and glycosylation, the protein migrates to 40-47 kDa, 50-55 kDa and 75-95 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 90% as determined by Bis-Tris PAGE
> 90% as determined by HPLC
Formulation
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
