Synonym
Replicase polyprotein 1a; pp1a; nsp1; PL2-PRO; PL-PRO; Papain-like Protease; Plpro
Source / expression
Recombinant SARS-CoV-2 PLpro/papain-like protease Protein is expressed from E.coil with His tag and Avi tag at the N-Terminus.It contains Glu1-Ile314 [Accession | P0C6U8].
Molecular weight
The protein has a predicted MW of 38.6 kDa same as Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 85% as determined by HPLC
Formulation
Supplied as 0.22μm filtered solution in 20mM Tris, 100mM NaCl, 10% Glycerol (pH 7.8).
Storage
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
