Synonym
Post-fusion glycoprotein F0;
Source / expression
Recombinant HRSV (B, strain 18537) Post-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus
Molecular weight
The protein has a predicted MW of 55.96 kDa. The protein migrates to 18-25 kDa and 42-50 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
Formulation
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
