Synonym
Cell surface-binding protein; Carbonic anhydrase homolog
Source / expression
Recombinant Monkeypox virus E8L Protein is expressed from E.coli with His tag at the N-Terminus.It contains Met1-Thr275 [Accession | QJQ40248.1].
Molecular weight
The protein has a predicted MW of 32.92 kDa. The protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
Formulation
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
